費繼鋒檢視原始碼討論檢視歷史

費繼鋒

華南師範大學腦科學與康復醫學研究院

費繼鋒 研究員

教育經歷

09.1991－07.1994 山東省章丘市第七中學

09.1994－07.1998 山東省萊陽農學院，植保專業，獲農學學士學位

09.1998－07.2001 華南農業大學，植物病理專業， 植物病毒學方向，導師，肖火根，李華平教授，獲農學碩士學位

09.2004－12.2008 德國馬克斯-普朗克分子細胞生物學與遺傳學研究所,(Max-Planck Institute of Molecular Cell Biology and Genetics) 導師Wieland B. Huttner 教授，所長，獲博士學位

工作經歷

09.2001－08.2002 新加坡分子農業研究所 (Institute of Molecular Agrobiology) 蔡南海教授實驗室, 助理研究官

08.2002－08.2004 新加坡淡馬錫生命科學研究院(Temasek Life Science Laboratory) 蔡南海教授實驗室, 助理研究官

01.2009－08.2009 德國馬克斯-普朗克分子細胞生物學與遺傳學研究所 (Max-Planck Institute of Molecular Cell Biology and Genetics)， Wieland B. Huttner 教授(所長)實驗室，博士後研究員

09.2009－10.2016 德國德累斯頓再生治療中心 (Center for Regenerative Therapies

Dresden)，Elly M. Tanaka 教授(所長)實驗室，博士後研究員

11.2016－現在 華南師範大學腦科學研究院，PI（副教授，實驗室主任），建立並主持神 經系統發育與再生實驗室

發表論文：

Nowoshilow S#, Schloissnig S#, Fei JF# (co-first author), Dahl A, Pang AWC, Pippel M, Winkler S, Hastie AR, Young G, Roscito JG, Falcon F, Knapp D, Powell S, Cruz A, Cao H, Habermann B, Hiller M*, Tanaka EM* and Myers EM*. The axolotl genome and the evolution of key tissue formation regulators. Nature. 2018 Feb 1;554(7690):50-55. doi: 10.1038/nature25458. (封面文章)

Fei JF*, Schuez M, Knapp D, Taniguchi Y, Drechsel D and Tanaka EM*. Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration. PNAS. 2017, 114(47):12501-12506. doi: 10.1073/pnas.1706855114. (封面文章)

Tazaki A, Tanaka EM and Fei JF. Salamander spinal cord regeneration: the ultimate positive control in vertebrate spinal cord regeneration. Developmental Biology. 2017, 432(1):63-71. doi: 10.1016/j.ydbio.2017.09.034.

Mircetic J, Steinebrunner I, Ding L, Fei JF, Bogdanova A, Drechsel D and Buchholz F. Purified Cas9 Fusion Proteins for Advanced Genome Manipulation. Small Methods.

2017, 1(4):1600052. doi: 10.1002/smtd.201600052

Fei JF*, Knapp D, Schuez M, Murawala P, Zou Y, Drechsel D and Tanaka EM*. Tissue- and time-directed electroporation of Cas9 protein-gRNA complexes in vivo yields efficient multigene knockout for studying gene function in regeneration. npj Regenerative Medicine. 2016,1(16002) ; doi:10.1038/npjregenmed.2016.2

Papanikos F#, Daniel K#, Ramlal A#, Fei JF, Kurth T, Wojtasz L, Derely I, Maiwald N, Penninger J, Surani A, Toth A*. The enigmatic meiotic dense body and its newly discovered component, SCML1, are dispensible for fertility and gametogenesis in mice. Chromosoma. 2017, 126(3): 399-415. doi:10.1007/s00412-016-0598-1

Wong FK#, Fei JF#(co-first author), Mora-Bermúdez F, Taverna E, Haffner C, Fu J, Anastassiadis K, Stewart AF and Huttner WB. Sustained Pax6 expression generates primate-like basal radial glia in developing mouse neocortex. PLOS Biology. 2015 Aug 7;13 (8):e10022172015.

Fei JF, Schuez M, Tazaki A, Taniguchi Y, Roensch K, Tanaka EM. CRISPR-Mediated Genomic Deletion of Sox2 in the Axolotl Shows a Requirement in Spinal Cord Neural Stem Cell Amplification during Tail Regeneration. Stem Cell Reports. 2014.Sep 9;3(3):444-59. (封面文章)

Fei JF, Haffner C, Huttner WB. 3' UTR-dependent, miR-92-mediated restriction of Tis21 expression maintains asymmetric neural stem cell division to ensure proper neocortex size. Cell Reports. 2014,7(2):398-411.

Fei JF, Huttner WB. Nonselective sister chromatid segregation in mouse embryonic neocortical precursor cells. Cerebral Cortex. 2009.Jul;19 Suppl 1:i49-54.

Pulvers JN, Schenk J, Arai Y, Fei JF, Saito K, Huttner WB. On the origin of neurons. Genome Biology. 2007.8(7): 311.

De Pietri Tonelli D, Calegari F, Fei JF, Nomura T, Osumi N, Heisenberg CP, Huttner WB. Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. Biotechniques. 2006. 41(6): 727-32.

Guo HS, Xie Q, Fei JF, Chua NH. MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development. Plant Cell. 2005.17(5):1376-86.

Guo HS, Fei JF, Xie Q and Chua NH. A chemical-regulated inducible RNAi system in plants. Plant Journal. 2003. 34, 383-392.

投稿論文：

Fei JF*, Lou WPK, Knapp D, Khattak S and Tanaka EM. Application and optimization of CRISPR/Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum). Nature Protocols. Revision

受邀會議報告：

09. 2008 45th Annual Meeting of the Society of Laboratory Animal Sciences and

the 10th Advanced Training Course of the IGTp

Title: "A Mouse Model With Inducible Cre Activity Restricted To

Neuron-Generating Progenitors" 大會報告

04. 2015 Zentrale Tierexperimentelle Einrichtung, medical school, University of Muenster

Title: "Developing Molecular Genetics in the Axolotl - a classical model for regeneration research" 特邀報告

09. 2015 Sino-German joint symposium in Beijing - Development and Maintenance of Brain Function:From Basic Mechanisms to Disease

Title: "Bonafide neural stem cell regeneration in axolotl- the promise of repairing injured mammalian CNS" 大會報告

10. 2017 中國細胞生物學學會第二屆模式動物器官再生研討會，北京

Title: "Development of modern genome engineering approaches and their applications in axolotl" 大會報告

教學經歷：

理論實踐課

05.2000 華南農業大學資源環境學院講授本科班「植物病毒學」10學時

11.2014 組織講授馬普所-德累斯頓工業大學博士班為期兩周的理論實踐課，

主題「研究Hippo信號通路在蠑螈脊髓再生過程中對神經幹細胞增殖／分化 的調控作用」

指導碩士／博士論文

2010年 指導碩士研究生Keith Gunapala，現為巴塞爾大學博士生

論文題目 "Investigating the trigger signal of axolotl spinal cord regeneration"

2012年 指導碩士研究生Björn Neumann，現為劍橋大學博士生

論文題目 "Development of knockdown approaches for gene function

analysis in Axolotls"

2013年 指導碩士研究生Dorothee Mugele，現為倫敦國王學院博士生

論文題目 "Evaluation of the RNAi-mediated gene knockdown in axolotls"

2015年 指導DAAD 獎學金學生Stephanie Marcus, 2016級洛克菲勒大學博士生

論文題目: CRISPR-mediated gene knockout as a method for studying regeneration of the spinal cord

2014-now 參與指導博士研究生Sergej Nowoshilow, 現為Elly Tanaka 實驗室博士生

研究基金項目：

2018-2019 國家自然科學基金面上項目 "利用再生模式生物蠑螈(Ambystoma

mexicanum)研究啟動脊髓再生的機制" (批准號：31771611)

項目負責人, 25萬元， 執行期限 2018年1月至2019年12月

2017-2019 華南師範大學高水平大學建設專項資金高層次引進人才科研啟動經費

項目負責人, 300萬元

2015-2016 CRTD postdoc seed grant "Inducible Cas9/CRISPR for Conditional Gene Knockouts in Vertebrate Regenerative Model Systems "

項目負責人, 1萬歐元

2014-2017 DFG grant (DFG274/3-3) "Establishment and application of targeted gene knockout and knock-in approach in axolotl for

regeneration research"

項目參與人, 30萬歐元