Kiryl D. Piatkevich檢視原始碼討論檢視歷史

Kiryl D. Piatkevich

來自 西湖大學 的圖片

本名 Kiryl D. Piatkevich 國家 中國 外語

Kiryl D. Piatkevich，是中國西湖大學著名的研究員、博士生導師。

簡介

Kiryl Piatkevich為西湖大學生命科學學院助理教授。從幼年開始，Piatkevich博士就對化學產生了極大的興趣。出於對科學的熱情，他15歲於白俄羅斯國立大學Lyceum開始學習化學專業。

從Lyceum畢業後，Piatkevich博士繼續在羅蒙諾索夫莫斯科國立大學攻讀碩士學位，同時從事有機金屬、分析化學、物理化學和生物有機化學等研究。在獲得榮譽理學碩士學位後，Piatkevich博士成功獲得繼續攻讀莫斯科國立大學與紐約阿爾伯特愛因斯坦醫學院聯合培養博士的機會，致力於開發小型哺乳動物活體成像的創新方法。

獲得化學博士學位後，Piatkevich博士作為博士後研究員加入麻省理工學院光遺傳學和神經工程學先驅Edward Boyden博士領導的合成神經生物學實驗室。在此期間，Piatkevich博士致力於利用合成生物學研發神經接口的新方法，並研發出了幾種新的分子技術用於觀察活體動物的神經元活動。

Piatkevich博士是最早在主要神經科學模式生物（包括蠕蟲、魚和小鼠）中以高時間精度對神經元電壓活動進行光學記錄的科學家之一。

從2019年開始，Piatkevich博士在西湖大學生命科學學院獨立領導分子生物工程實驗室（實驗室網址：www.piatkevich-lab.com）。

學術成果

Piatkevich博士的研究方向是研發和設計尖端分子和成像技術，用於分析、控制和修復複雜生物系統，如大腦。設計用於光學記錄和操控神經元活動的遺傳編碼分子工具，以及用於神經元解剖學追蹤的熒光探針。這是一個跨學科研究計劃，涵蓋合成和化學生物學、生物工程、生物化學、生物技術、分子體內成像、神經科學、機器人和細胞生物學等領域。Piatkevich實驗室將系統地應用其研發的技術來揭示腦紊亂的分子機制並揭示神經編碼的基本原理。新的分子技術也會用於通過光創建先進的腦機接口。

代表論文

1.Piatkevich K.D.#, Bensussen S.#, Tseng H.#, Shroff S.N., Lopez-Huerta V.G., Park D., Jung E.E., Shemesh O.A., Straub C., Gritton H.J., Romano M.F, Costa E., Sabatini B.L., Fu Z, Boyden E.S., Han X. Population imaging of neural activity in awake behaving mice.Nature 2019,in press(# equal contribution).
　　2. Qian Y.#,Piatkevich K.D.#, McLarney B.#, Abdelfattah A.S., Mehta S., Gottschalk S., Molina R.S., Zhang W., Drobizhev M., Hughes T.E., Zhang J., Schreiter E.R., Shoham S., Razansky D., Boyden E.S., Campbell R.E. A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator.Nature Methods, 16 (2), 171 (# equal contribution).
　　3.Piatkevich K.D.#, Jung E.E.#, Straub C., Linghu C., Park D., Suk H.J., Hochbaum D.R., Goodwin D., Pnevmatikakis E., Pak N., Kawashima T., Yang C.-T., Rhoades J.L., Shemesh O., Asano S., Yoon Y.-G., Freifeld L., Saulnier J., Riegler C., Engert F., Hughes T., Drobizhev M., Szabo B., Ahrens M.B., Flavell S.W., Sabatini B.L., Boyden E.S. A Robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.Nature Chemical Biology, 14 (4), 352 (# equal contribution).
　　4.Piatkevich K.D., Suk H.J., Suhasa B.K., Yoshida F., DeGennaro E.M., Dro bizhev M., Hughes T.E., Desimone R., Boyden E.S.*, Verkhusha V.V. Near-infrared fluorescence proteins engineered from bacterial phytochromes in neuroimaging.Biophys. J. 113(10), 2299-2309.
　　5.Piatkevich K.D., English B.P., Malashkevich V.N., Xiao H., Almo S.C., Singer R.H., Verkhusha V.V. Photoswitchable red fluorescent protein with a large Stokes shift.Chem Biol.2014. 21:1402.
　　6.Piatkevich K.D.*, Murdock M.H., Subach F.V.* Advances in engineering and application of optogenetic indicators for neuroscience.Applied Sciences. 2019, 9(3), 562. (*co-corresponding).
　　7. Tillberg P.W.#, Chen F.#,Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L., Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D., Boyden E.S. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.Nature Biotechnology. 34 (9), 987-992 (# equal contribution).
　　8. Shemesh O.A., Tanese D., Zampini V., Linghu C.,Piatkevich K., Ronzitti E., Papagiakoumou E., Boyden E.S., Emiliani V. Temporally precise single-cell resolution optogenetics.Nature Neuroscience. 20(12), 1796-1806.
　　9. Subach O.M, Barykina N.V., Anokhin K.V.,Piatkevich K.D., Subach F.V. Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome.Int. J. Mol. Sci., 20(14), 3488.
　　10. Subach O.M., Kunitsyna T.A., Mineyeva O.A., Lazutkin A.A., Bezryadnov D.V., Barykina N.V.,Piatkevich K.D., Ermakova Y.G., Bilan D.S., Belousov V.V., Anokhin K.V., EnikolopovG.N.*, Subach F.V.* Slowly reducible genetically encoded green fluorescent indicator for in vivo and ex vivo visualization of hydrogen peroxide.Int. J. Mol. Sci.20(13), 3138;
　　11. Barykina N.V., Doronin D.A., Subach O.M., Sotskov V.P., Plusnin V.V., Ivleva O.A., Gruzdeva A.M., Kunitsyna T.A., Ivashkina O.I., Lazutkin A.A., Malyshev A.Y., Smirnov I.V., Varizhuk A.M., Pozmogova G.E.,Piatkevich K.D., Anokhin K.V., Enikolopov G.N., Subach F.V*. NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.Scientific Reports, 8 (1), 15233.
　　12. Doronin D., Barykina N., Subach O., Isaakova E., Varizhuk A., Pozmogova G.,Piatkevich K., Anokhin K., Enikolopov G., Subach F.* Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast.BMC Biotechnology, 18 (1), 10.
　　13. Barykina N.V., Subach O.M.,Piatkevich K.D., Jung E.E., Malyshev A.Y., Smirnov I.V., Bogorodskiy A.O., Borshchevskiy V.I., Varizhuk A.M., Pozmogova G.E., Boyden E.S., Anokhin K.V., Enikolopov G.N., Subach F.V.*. Green fluorescent genetically encoded calcium indicator based on calmodulin/M13-peptide from fungi.PLoS One.12(8):e0183757.
　　14. Rowlands C.J., Park D., Bruns O.T.,Piatkevich K.D., Fukumura D., Jain R.K., Bawendi M.G., Boyden E.S., So P.T.C.*. Wide-field three-photon excitation in biological samples.Light: Science & Applications. 6, e16255.
　　15. Barykina N.V., Subach O.M., Doronin D.A., Sotskov V.P., Roshchina M.A., Kunitsyna T.A., Malyshev A.Y., Smirnov I.V., Azieva A.M., Sokolov I.S.,Piatkevich K.D., Burtsev M.S., Varizhuk A.M., Pozmogova G.E., Anokhin K.V., Subach F.V.*, Enikolopov G.N.*. A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites.Scientific Reports.6:34447.
　　16. Konold P.E., Yoon E., Lee J., Allen S.L., Chapagain P.P., Gerstman B.S., Regmi C.K.,Piatkevich K.D., Verkhusha V.V., Joo T., Jimenez R.*. Fluorescence from multiple chromophore hydrogen-bonding states in the far-red protein TagRFP675.J. Phys. Chem. Lett.7, 3046-3051.