羅暉 （教授）檢視原始碼討論檢視歷史
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羅暉，男，北京科技大學化學與生物工程學院教授。
人物簡歷
1、2009-2010麻省理工學院訪問學者 2、2004年至今 北京科技大學教師 3、2004年清華大學獲生物化工博士學位 4、1999年北京化工大學獲生物化工碩士學位 5、1996年北京化工大學獲生物化工學士學位
科研方向
社會職務
Biotechnology Notes編委
科研業績
1、主持和完成國家自然科學基金項目3項； 2、主持和完成「北京科技新星」計劃項目； 3、參與和完成國家科技支撐計劃子課題、國家高技術研究發展計劃（863計劃）課題、國家重點基礎研究發展計劃（973計劃）子課題、北京市自然科學基金項目多項； 4、承擔企業合作課題多項。
獲得獎勵
1、獲得國家發明二等獎（排名第3）1項，中國高校科技發明二等獎（排名第3）1項、中國石油和化工協會科技進步二等獎（排名第3）1項； 2、羅暉*，劉秀紅，常雁紅，彭欽成，李璽，何華，于慧敏，沈忠耀. 7-氨基頭孢烷酸的製備方法. 申請號：201410612. 3、羅暉*，韓凱華，常雁紅，魏艷梅，何華，于慧敏，李強，沈忠耀. 一種固定化頭孢菌素C酰化酶的製備方法. 申請號：201310276375.6. (專利) 4、羅暉*，何華，常雁紅，魏艷梅，于慧敏，沈忠耀. 一種製備固定化頭孢菌素C酰化酶的方法. 申請號：201310401009.9. 5、羅暉*，紀欽洪，常雁紅，杜翠鳳，于慧敏，沈忠耀. 一種融合蛋白及其編碼基因和用途. 申請號：200710177396.7 6、羅暉*，尹祖建，常雁紅，肖寶清. 一種催化煙酸製備6－羥基煙酸的方法及其所用菌株. 專利號：200710177394.8 羅暉*，史芫芫，于慧敏，李強，沈忠耀. 一種戊二酰-7-氨基頭孢烷酸酰化酶的固定化方法.中國專利，專利號：200610011742.X.
學術成果
論文論著
1.Tian J, Jia R, Dong W, Sun H, Wang Y, Chang Y, Luo H*. (2021). One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry. Biotechnology Letters, 43(5), 1075-1087.
2.Sun H, Liu T, Luo H*, Nie Z, Chang Y, Yu H, Shen Z. Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library. Applied Biochemistry and Biotechnology, 2021, 193(4):1056-1071.
3.Liu J, Tong S, Sun H, Chang Y, Luo H*, Yu H, Shen Z. Effect of shaking speed on immobilization of cephalosporin C acylase: Correlation between protein distribution and properties of the immobilized enzymes. Biotechnology Progress. 2020, 37(1):e3063.
4.Wang Y, Chang Y, Jia R, Sun H, Tian J, Luo H*, Yu H, Shen Z. SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability. Process Biochemistry, 2020, 95:260-268.
5.Nie Z, Luo H*, Li J, Sun H, Xiao Y, Liu T, Chang Y, Yu H, Shen Z. High-throughput screening of T7 promoter mutants for soluble expression of cephalosporin C acylase in E. coli. Applied Biochemistry and Biotechnology, 2020, 190:293-304.
6.Chang Y, Tong S, Luo H*, Liu Z, Qin B, Zhu L, Sun H, Yu H, Shen Z. (2019). Application of ammonium bicarbonate buffer as a smart microenvironmental pH regulator of immobilized cephalosporin C acylase catalysis in different reactors. Biotechnology Progress. 2019, 35(5):e2846.
7.Wang Y, Tian J, Xiao Y, Wang Y, Sun H, Chang Y, Luo H*. SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase. Biotechnology Letters, 2019, 41(8-9):987-994.
8.Luo H*, Han K, Chang Y, Wang Y, Tong S, Nie Z, Wang X, Wei Y, Yu H, Shen Z. Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase. Chem. Biochem. Eng. Q., 2018, 32(1):125-133.
9.Luo H*, Zhu L, Chang Y, Liu X, Liu Z, Sun H, Li X, Yu H, Shen, Z. Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process. Bioresource Technology, 2017, 223:157-165.
10.Xu J, Luo H*, López C, Xiao J, Chang Y*. Novel immobilization process of a thermophilic catalase: efficient purification by heat treatment and subsequent immobilization at high temperature. Bioprocess and Biosystems Engineering, Bioprocess Biosyst Eng, 2015, 38:1983-1991.
11.Luo H*, Ma J, Chang Y, Yu H, Shen Z. Directed evolution and mutant characterization of nitrilase from Rhodococcus rhodochrous tg1-A6. Appl Biochem Biotechnol, 2016, 178(8):1510-1521.
12.Luo H*, Zhao H, Chang Y, Wang Q, Yu H, Shen Z. Oriented immobilization and characterization of a poly-lysine tagged cephalosporin C acylase on glyoxyl agarose support. Appl Biochem Biotechnol, 2015, 175:2114-2123.
13.He H, Wei Y, Luo H*, Chang Y, Yu H, Shen Z. Immobilization and stabilization of cephalosporin C acylase on aminated support by cross-linking with glutaraldehyde and post-modification using aminated macromolecules. Biotechnology Progress, 2015, 31(2):387-95.
14.Wei Y, Luo H*, Chang Y, Yu H, Shen Z. Reversible immobilization of cephalosporin C acylase on epoxy supports coated with polyethyleneimine. Biocatalysis and Biotransformation, 2015, 33(5-6):250-259.[1]