檢視安利佳的原始碼

[[File:应1385.jpg|缩略图|[http://faculty.dlut.edu.cn/1998011004/zh_CN/index.htm 原图链接]]]
'''张西文''',男，现任中国遗传学会理事，中国化工学会生物工程分会理事，教育部科委生命科学学部委员，辽宁省遗传学会及生物技术学会理事。1998-2002年任大连理工大学生物工程系主任主要从事植物基因工程和药物生物技术研究，先后主持国家科技攻关项目1项、省、市级重大研究课题4项。

==工作经历==

1998.3 至今[[大连理工大学[[ 教师

1984.10 1998.2[[辽宁师范大学]] 教师

1975.12 1978.2[[丹东市陶瓷厂]] 工人

1973.12 1975.12丹东市东沟县海洋红公社 知青

1972.12 1973.12丹东市凤城县赛马公社 知青

==教育经历==

1986.9至1989.8 [[东北师范大学]]植物学博士

1982.3至1984.8 东北师范大学遗传学  硕士

1978.3至1982.2 [[辽宁师范大学]]生物学学士

1969.9至1972.8[[丹东市第二中学]]高中课程
<ref>[http://faculty.dlut.edu.cn/1998011004/zh_CN/index.htm  大连理工大学] </ref>

==社会兼职编==

2006.5至今评委(国家留学基金委)

2007.10至今副理事长(中国园艺学会小浆果分会)

2005.12020.12学部委员(教育部科委生命科学学部)

2005.12020.12理事(中国遗传学会)

2004.122019.12专家(辽宁省生物技术协会专家委员会)

==研究领域==

1. 植物转基因工程与育种技术平台研究。编号：JY03-B-18，国家科技部植物转基因重大专项，100万元，2003-2004

2. 基因工程关键技术。项目编号：2001101001，辽宁省科技厅，210万元，2001-2004

3. 无载体、无标记基因植物转基因技术。项目编号：2003B4NS015，大连市科技局，100万元，2003-2004

4. 蛇岛蝮蛇类凝血酶基因的分离和表达。辽宁省科技攻关，10万元，1999.01-2001.12

5. 蛇岛蝮蛇类凝血酶基因的分离和表达。大连市科委，15万元，1998-2001

6. 胸腺素α1基因工程药物开发。通化金马制药股份公司，390万元，1998.01~2001.12

7. 杨树基因工程育种。鞍山生生高新植物技术应用股份有限公司，25万元，2001.9-2003.9

8. 长春花碱单体的分离研究。大连理工大学领先集团有限公司，20万元，2002.7-2003.7

9. 真福康保健功效的验证与鉴定。大连理工大学领先集团有限公司，10万元，2002.7-2003.7

==指导硕、博士生研究方向==

植物基因工程、生物制药

==出版著作==

五年来共发表文章120余篇，SCI收录42篇，主要文章包括：

1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry,2005,40:213–216　SCI

2. Purification,characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii 
ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI

3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response,therapeutic 
time-window and long-term efficacy. Brain Research,2004,1029:179-185　SCI

4. Plasmodium falciparum histone acetyltransferase,a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic 
Cell,2004,3（2）：264-276

5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and 
inactivating of caspase casecade. Toxicon,2004,43:53-59

6. PfADA2,a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with 
the histone acetyltransferase PfGCN5. Gene,2004,336:251-261

7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm,Antheraea pernyi. DNA 
and Cell Biology. 2003,22:441-446

8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of 
Biosciences,2003,28（6）：691-695

9. 菟丝子提取物在PC12细胞株中的神经营养因子样活性。生物化学与生物物理进展。2003,30（2）：226-230

10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in 
PC12 cells. Acta Pharmacologica Sinica,2003,24（9）：913-917

11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. 
International Journal of Developmental Neuroscience,2003,21:277-281

12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology 
International,2003,27:1025-1031

13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. 
Toxicology Letters,2003,144（2）：205–212

14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical 
neurons. Neurosci Res Commun,2003,33（2）：105–113

15. Investigation of the neuronal death mode induced by glutamate treatment in serum-,antioxidant-free primary cultured 
cortical neurons. Developmental Brain Research,2003,145（2）：263-268

16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science 
Bulletin,2003,48（16）：1718-1721

17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae 
by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters,2003,25:1191-1194

18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of 
microglial activation. The Journal Of Pharmacology and Experimental Therapeutics,2003,305（1）：212-218

19. 酸性水溶液中硫氰酸钼(V)配合物的研究。光谱学与光谱分析，2003,23（2）337-339

20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & 
Biotechnology,2003,33（2）：113-123

21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. 
Acta Botanica Sinica,2003,45（2）：242-247

22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use 
in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25（17）：1431-1436

23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine 
Journal,2003,116（2）：239-242

24. Expression of gloshedobin,a thrombin-like enzyme from the venom of Gloydius shedaoensis,in Escherichia coli. 
Biotechnology Letters,2003,25（2）：101–104

25. High-level expression of a soluble snake venom enzyme,gloshedobin,in E. coli in the presence of metal ions. 
Biotechnology Letters,2003,25（8）：607–610

26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh 
root of Panax ginseng. Process Biochemistry,2002,37:793-798

27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters,2002,24:135-
138

28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius 
ussuriensis. Prog Biochem Biophys. 2002,34（1）：6-10

29. Expression,Purification and Partial Characterization of Recombinant Defibrase,a Thrombin-Like Enzyme from the Venom 
of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29（3）390-393

30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 
2002,24（18）：1531-1534

31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology 
Reporter,2002,20:189a-189e

32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11（2-
3）：79-85

33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large 
na&iuml;ve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29
（4）：572-575

34. Inhibition by naloxone stereoisomers of beta-amyloid peptide （1-42）-induced superoxide production in microglia 
and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics,2002,302
（3）：1212-1219

35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and 
Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22（2）：197-206

36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical 
significance. Chinese Medical Journal,2002,115（11）：1615-1619

37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. 
Chinese Science Bulletin,2002,47（21）：1811-1815

38. 铜锌超氧化物歧化酶与色氨酸钴（Ⅱ）的相互作用。光谱学与光谱分析，2000,3:305-307

39. 紫外-可见吸收光谱法测定配合显色反应的焓变。光谱学与光谱分析，2001,21（4）：527-528

40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. 
Journal of Enzyme Inhibition,2001,16:313-319

41. Purification and Characterization of ginsenoside-?-Glucosidase from Ginseng. Chem. Pharm. Bull,2001,49（7）：795-
798

42. 分光光度法研究EDTA对小牛肠碱性磷酸酶的不可逆抑制动力。光谱学与光谱分析，2001,21（5）：701-703

43. 碱性磷酸梅与Cu （Ⅱ） 相互作用的谱学研究。光谱学与光谱分析，2001,21（4）：432-434

44. 氨基酰化酶与Cu（Ⅱ）相互作用的谱学研究。光谱学与光谱分析，2001,21（6）：804-806

45. 可见光谱学研究核酸酶P1与氯化铜（Ⅱ）的相互作用。光谱学与光谱分析，2000,20（3）：308-310

46. 大连蛇岛蝮蛇类凝血酶基因克隆与表达研究。大连理工大学学报，2003,43（2）：156-159

47. 新的重组蛋白表达系统——植物根分泌和叶分泌。生物化学与生物物理进展，2003,30（1）：128

48. 抗乙肝病毒表面抗原PreS1（20-47）的单链抗体在转基因烟草根分泌物中的初步表达。高技术通讯，2003,13（9）：35-38

49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm,Antheraea pernyi. High 
Technology Letters,2003,9（3）：29-32

50. 喜树碱生物合成途径及其代谢调控的研究进展。高技术通讯，2004,14（5）：106-110

51. 菟丝子提取物对PC12细胞中GAP-43表达影响。大连理工大学学报，2004,44（3）：378-382

52. Rapid Amplification of 5′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High 
Technol Lett　2002,8（1）：5-7

53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology 
Letters,2002,8（2）：5-17

54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High 
Technology Letters,2002,8（3）：5-10

55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High 
Technology Letters,2002,8（2）：5-10

56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters,2002,8（3）：1-4

57. 精子干细胞转染法制备转基因兔的研究。高技术通讯，2001,11（130）：17-21

58. 反向嵌套PCR高效扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 5’末端序列。高技术通讯，2001,1（11）：17-19

59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology 
Letters,2001,7（3）：8-10

60. 新型染料配基对碱性磷酸酶的亲合纯化。高校化学工程学报，2001,5（6）：563-567

61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters，2001,7（4）：
13-15

62. 卵黄免疫球蛋白配基亲和纯化碱性磷酸酶。中国农业科学，2004,37（6）：908-911

==参考资料== 
{{Reflist}}

[[Category:教师]]