林娟（教授）
人物簡歷
1988.9-1992.7：華東師範大學生物系，大學本科；
1998.9-2001.7：福州大學生物化工專業，碩士研究生；
2003.9-2007.4：福州大學分析化學專業，博士研究生；
1992.7-1995.4：福建農林大學；
1995.5-今：福州大學生物科學與工程學院.
研究方向
社會兼職
中國生物化學與分子生物學會酶學專業分會委員，福建省生物化學與分子生物學會副理事長；福建省微生物學會常務理事；福建省水產學會理事。
科研項目
1、 D-對甲碸基苯絲氨酸乙酯（D-乙酯）的產業化生產（2022-2026），1000萬；
2、 工業屬性高效生物催化劑的設計與開發（2019-2023），300萬；
3、 甾醇類化合物酶催化技術開發（2022-2025），120萬；
4、 生物發酵功能飼料的開發（2019-2022），60萬；
5、 福建省基金重點項目「環己酮單加氧酶不對稱氧化奧美拉唑硫醚的分子機理研究」（2021-2024），30萬；
6、 國家科技部863子課題「新型糖酶創製與創新應用關鍵技術研究」（2013-2017），69.3萬；
7、 國家海洋局海洋公益性行業科研專項子課題「海洋生物酶資源庫及幾種海洋源性工業用酶關鍵生產技術開發」（2013-2016）；
8、 福建省高校產學合作重大項目「利用現代微生物工程技術發酵生產超氧化物歧化酶」（2013-2016），50萬；
9、 福建省高校產學合作重大項目「高色價紅曲紅色素液態深層發酵關鍵技術開發」（2017-2020），40萬；
10、 福建省發改委生物產業項目「漆酶的產業化生產技術開發」（2011-2014），30萬；
11、 福建省經信委企業技術創新項目「海洋藻類資源高值化利用技術創新平台建設」（2015-2017），50萬；
12、 福建省海洋高新產業發展專項「魚罐頭加工廢棄液的高值化利用」（2015-2017），50萬。
學術成果
論文
1. Construction of an Efficient Non-natural Enzyme System for Preparation of Testosterone in High Space-Time Yield. ACS Sustainable Chemistry & Engineering, 2022.（Top 期刊）
2. 「Nonpolarity paving」 in substrate tunnel of a Limnobacter sp. Phenylacetone monooxygenase for efficient single whole-cell synthesis of esomeprazole. Bioorganic Chemistry, 2022.（Top 期刊）
3. An Effective Chemo-Enzymatic method with An Evolved L-Threonine Aldolase for Preparing L-threo-4-Methylsulfonylphenylserine Ethyl Ester of High Optical Purity. Molecular Catalysis, 2022.
4. Engineered Cyclohexylamine Oxidase with Improved Activity and Stereoselectivity for Asymmetric Synthesis of a Bulky Dextromethorphan Precursor and Its Analogues. ChemCatChem, 2022.
5. Construction of efficient enzyme systems for preparing chiral ethyl 3-hydroxy-3-phenylpropionate. Enzyme and Microbial Technology, 2022.
6. Efficient biosynthesis of (2S, 3R)-4-methylsulfonylphenylserine by artificial self-assembly of enzyme complex combined with an intensified acetaldehyde elimination system. Bioorganic Chemistry, 2021.（Top 期刊）
7. Improving the Cβ Stereoselectivity of l-Threonine Aldolase for the Synthesis of l-threo-4-Methylsulfonylphenylserine by Modulating the Substrate-Binding Pocket To Control the Orientation of the Substrate Entrance. Chemistry—A European Journal, 2021.（Top 期刊）
8. Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability. Enzyme and Microbial Technology, 2021.
9. Single-Point Mutation Near Active Center Increases Substrate Affinity of Alginate Lyase AlgL-CD. Applied Biochemistry and Biotechnology, 2021.
10. Multi-enzyme cascade for improving ß-hydroxy-a-amino acids production by engineering L- threonine transaldolase and combining acetaldehyde elimination system. Bioresource Technology, 2020.（Top 期刊）
11. Asymmetric Synthesis of a Key Dextromethorphan Intermediate and Its Analogs Enabled by a New Cyclohexylamine Oxidase: Enzyme Discovery, Reaction Development, and Mechanistic Insight. Journal of Organic Chemistry, 2020.（Top 期刊）
12. A L-threonine aldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Chemical Engineering Science, 2020.（Top 期刊）
13. Expression and characterization of a chitinase from Serratia marcescens. Protein Expression and Purification, 2020.
14. Rational Design of Dehydrogenase/Reductases Based on Comparative Structural Analysis of Prereaction-State and Free-State Simulations for Efficient Asymmetric Reduction of Bulky Aryl Ketones. ACS Catalysis, 2019.（Top 期刊）
15. Characteristics of L-threonine transaldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Catalysis Science & Technology, 2019.（2019年熱點文章）
16. Truncation of κ‑carrageenase for higher κ‑carrageenan oligosaccharides yield with improved enzymatic characteristics. International Journal of Biological Macromolecules, 2019.（Top 期刊）
17. Mutagenesis on the surface of a β-agarase from Vibrio sp. ZC-1 increased its thermo-stability. Enzyme and Microbial Technology, 2019.
18. Inhibitory mechanism of Penicillin V on mushroom tyrosinase. Molecular Biology Reports, 2019.
19. Prediction of zinc-binding sites using multiple sequence profiles and machine learning methods. Molecular Omics, 2019.
20. Preparation of bioactive neoagaroligosaccharides through hydrolysis of Gracilaria lemaneiformis agar: A comparative study. Food Chemistry, 2018.（Top 期刊）
21. A Novel Multi-domain High Molecular, Salt-Stable Alkaline Xylanase from Alkalibacterium sp. SL3. Frontiers in Microbiology, 2017.（Top 期刊）
22. Inactivation kinetics and conformation change of Hypocrea orientalis b-glucosidase with guanidine hydrochloride. Journal of Bioscience and Bioengineering, 2017.
23. Degradation of tetracycline by immobilized laccase and the proposed transformation pathway. Journal of Hazardous Materials, 2017.（Top 期刊）
24. Immobilized Cerrena sp. Laccase preparation, thermal inactivation, and operational stability in malachite green decolorization. Scientific Reports, 2017.
25. Laccases Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation. Frontiers in Microbiology, 2017.（Top 期刊）
26. A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake. Scientific Reports, 2016.
27. Cross-linked enzyme aggregates of Cerrena laccase: Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive. Journal of the Taiwan Institute of Chemical Engineers, 2016.
28. Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with fungal laccase. Analytical Biochemistry, 2016.
29. Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp.in Response to Metal Ions, Aromatic Compounds, and Nutrients. Frontiers in Microbiology, 2016.（Top 期刊）
30. Optimal parameters for laccase-mediated destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels. Data in Brief, 2016.
31. A neural network learning approach for improving the prediction of residue depth based on sequencederived features. RSC Advances, 2016.
32. Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis. Molecules, 2016.
33. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris. Biotechnology and Applied Biochemistry, 2016.
34. The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Biochem Biophys Res Commun, 2016.
35. Laccase-Catalyzed Decolorization of Malachite Green: Performance Optimization and Degradation Mechanism. PLOS ONE, 2015.
36. Molecular characterization of a thermophilic and salt and alkaline-tolerant xylanase from Planococcus sp. SL4, a strain isolated from the sediment of a soda lake. Journal of Microbiology and Biotechnology（JMB）, 2015.
37. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris, Biotechnology and Applied Biochemistry, 2015.
38. A novel laccase from basidiomycete Cerrena sp.: Cloning, eterologousexpression, and characterization. International Journal of Biological Macromolecules, 2015.
39. Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability. PLOS ONE, 2014.
40. High Phylogenetic Diversity of Glycosyl Hydrolase Family 10 and 11 Xylanases in the Sediment of Lake Dabusu in China. PLOS ONE, 2014.
41. Antioxidant activities of kombucha prepared from three different substrates and changes in content of probiotics during storage. Food Science and Technology, 2014.
獲獎情況
教學：分別獲得福州大學第五屆、第六屆青年教師「最佳一節課」競賽優秀獎和二等獎，並獲得福建省教學成果二等獎1項，福州大學教學成果獎4項，7次獲得校教學優秀二等獎，2015年獲得福州大學廈航獎教金。
科研：獲省科技進步三等獎1項，2007年入選福建省高等學校新世紀優秀人才支持計劃。[1]