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王晓云
原图链接 图片来源360搜索 教授，博士生导师
出生	1962年6月
国籍	中国
职业	教育科研工作者
知名作品	《利用定点突变技术研究二硫键对植酸酶结构、催化活性及热稳定性的作用》

王晓云，女，毕业于山东农业大学，先后获得硕士和博士学位。2008年到2010年到美国哈佛大学做高级访问学者

人物经历

1983年山东农业大学本科毕业。

1985以来任教至今，先后获得山东农业大学硕士和博士学位。

2002-2003在清华大学做访问学者。

2008-2010年两度到美国哈佛大学做高级访问学者。

"Global Journal of Biochemistry"国际期刊编委。

"Comparative Biochemistry and Physiology" 审稿人。

"International Journal of Biological Macromolecules"审稿人。

"Journal of Molecular Catalysis"审稿人。

主讲课程

承担本科生《生物化学》、《分子生物学》、《基础生物化学实验》;硕士研究生《生物化学》;博士研究生《蛋白质结构与功能》等课程的教学工作，主持的生物化学课程为教育部双语建设课程、山东省首届精品课程和山东省重点建设课程，所在的教学团队为生物化学与分子生物学系列课程国家级教学团队。

研究方向

主要研究方向为蛋白质(酶)的结构与功能的关系，在克隆、表达基因的基础上，利用定点突变技术，研究关键氨基酸残基对蛋白质结构及功能的影响等，特别是逆境胁迫下蛋白质表达与氧化胁迫的关系，以及二硫键形成途径中关键酶的研究等，也包括酶的抑制动力学及生物体内代谢调控酶的分离纯化、性质研究及调控机理的研究。

一直以来从事生物化学与分子生物学、植物生理生化方面的科研与教学工作，着重研究酶的催化机制以及结构与功能的关系。主持承担国家自然基金、高等学校博士学科点专项科研基金、山东省优秀中青年科学家科研奖励基金、作物生物学国家重点实验室基金等课题。

主要贡献

作为第一作者或通讯作者在"Antioxidant and Redox Signalling"、"International Journal of Biochemistry and Cell Biology" 、"International Journal of Biological Macromolecules"、"中国农业科学"等国内外学术刊物发表学术论文60多篇。

科研项目

1.农业部公益性行业科研专项"我国重要野生果树资源的收集、评价与优异种质创新利用技术研究与示范"(201303093).。

2.国家自然科学基金《利用定点突变技术研究二硫键对植酸酶结构、催化活性及热稳定性的作用》(No.30471261)。

3.国家自然科学基金《通过引入二硫键、增加糖基化位点等方式提高植酸热稳定性的研究》(No.30940017)。

4.高等学校博士点专项基金:《糖基化及二硫键对植酸酶稳定性的研究》(No.20050434008)。

5.山东省中青年科学家科研奖励基金《叶片衰老关键酶分离纯化及调控衰老物质的研制与开发》 。

科研成果

对酶的热稳定性及二硫键在去折叠和再折叠过程中的作用，有较深入的研究，指导博士研究生6人，硕士研究生33人，作为第一作者或通讯作者，在"Antioxidant and Redox Signalling"、"International Journal of Biochemistry and Cell Biology" 、"International Journal of Biological Macromolecules"、"Journal of Integrative Plant Biology"、"Plant Science"、"中国农业科学"、"作物学报"等国内外学术刊物发表学术论文60多篇，其中SCI收录30余篇。

发表论文

Yu Z. B., Yang X. J., Du J. J., Wan C. M., Xu J. N., Wang W. J., Feng Y. G., and Wang X. Y.*, A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress. Physiol Plant. 2015. DOI:10.1111/ppl.12376.

Yang X. J., Cui H. R., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Key Amino Acids of Arabidopsis VKOR in the Activity of Phylloquinone Reduction and Disulfide Bond Formation. Protein Pept Lett. 2015, 22:81-86.

Xu J. N., Xing S. S., Cui H. R., Chen X. S., and Wang X. Y.*, Genome-wide identification and characterization of the apple (Malus domestica) HECT ubiquitin-protein ligase family and expression analysis of their responsiveness to abiotic stresses. Mol Genet Genomics. 2015. DOI:10.1007/s00438-015-1129-0.

Lu Y., Peng J. J., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Thylakoid membrane oxidoreductase LTO1/AtVKOR is involved in ABA-mediated response to osmotic stress in Arabidopsis. Physiol Plant. 2015, 154:28-38.

Lu Y., Du J. J., Yu Z. B., Peng J. J., Xu J. N., and Wang X. Y.*, Identification of potential targets for thylakoid oxidoreductase AtVKOR/LTO1 in chloroplasts. Protein Pept Lett. 2015, 22:219-225.

Li F., Li W., Liu Y., Tong Y. X., Zhou P., Wang L., Chen C., Zeng L. Y., Wu Q. Y., Wang X. Y. *, and Xu K. L., Effects of the I682F mutation on JAK2's activity, structure and stability. Int J Biol Macromol. 2015, 79:118-125.

Du J. J., Zhan C. Y., Lu Y., Cui H. R., and Wang X. Y.*, The conservative cysteines in transmembrane domain of AtVKOR/LTO1 are critical for photosynthetic growth and photosystem II activity in Arabidopsis. Frontiers in Plant Science. 2015, 6:238. Cui H. R., Zhang Z. R., Lv W., Xu J. N., and Wang X. Y.*, Genome-wide characterization and analysis of F-box protein-encoding genes in the Malus domestica genome. Molecular Genetics and Genomics. 2015, 290:1-12.

Jing X. L., Xu J., Fan M. N., Ma L., Huang X., Wang X. Y., Sun S. H., Zhu C. X., and Liu H. M., Mutation analysis of the RNA silencing suppressor NS1 encoded by avian influenza virus H9N2. Journal of General Virology. 2015, 96:1613-1618.

Zhan C. Y., Xu J. N., Li D., Liu B. B., Feng Y. G., and Wang X. Y. *, Overexpression of AtCBF1 in Sinningia speciosa Enhances Chilling Stress Tolerance. Research & Reviews: Journal of Botanical Sciences. 2015, S1:26-33.

Wu Q. Y., Guo H. Y., Geng H. L., Ru B. M., Cao J., Chen C., Zeng L. Y., Wang X. Y., Li F., and Xu K. L., T273 plays an important role in the activity and structural stability of arginine kinase. Int J Biol Macromol. 2014, 63:21-28.

Wang P., Li X. Z., Cui H. R., Feng Y. G., and Wang X. Y*., Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum. Plant Physiol Biochem. 2014, 76C:58-66.

Wan C. M., Yang X. J., Du J. J., Lu Y., Yu Z. B., Feng Y. G., and Wang X. Y*., Identification and characterization of SlVKOR, a disulfide bond formation protein from Solanum lycopersicum, and bioinformatic analysis of plant VKORs. Biochemistry (Mosc). 2014, 79:440-449.

Geng H. L., Bian M. R., Liu Y., Cao J., Chen C., Wang Z. Y., Li Z. Y., Zeng L. Y., Wang X. Y. *, Wu Q. Y., and Xu K. L., The D14 and R138 ion pair is involved in dimeric arginine kinase activity, structural stability and folding. Int J Biol Macromol. 2014, 66:302-310.

Du F., Xu J. N., Zhan C. Y., Yu Z. B., and Wang X. Y.*, An obesity-like gene MdTLP7 from apple (Malus× domestica) enhances abiotic stress tolerance. Biochemical and biophysical research communications. 2014, 445:394-397.

Du F., Xu J. N., Li D., and Wang X. Y.*, The identification of novel and differentially expressed apple-tree genes under low-temperature stress using high-throughput Illumina sequencing. Mol Biol Rep. 2014, 42:569-580.

Yu Z. B., Lu Y., Du J. J., Peng J. J., and Wang X. Y.*, The chloroplast protein LTO1/AtVKOR is involved in the xanthophyll cycle and the acceleration of D1 protein degradation. J Photochem Photobiol B. 2013, 130C:68-75.

Lu Y., Wang H. R., Li H., Cui H. R., Feng Y. G., and Wang X. Y*., A chloroplast membrane protein LTO1/AtVKOR involving in redox regulation and ROS homeostasis. Plant Cell Rep. 2013, 32:1427-1440.

Li F., Wu Q. Y., and Wang X. Y., The amino acid residue L113 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2013, 52:198-205.

Wu Q. Y., Li F., Wang X. Y. *, and Xu K. L., Evidence that the amino acid residue Ile121 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2012, 51:369-377.

Wu Q. Y., Li F., Guo H. Y., Cao J., Chen C., Chen W., Zhao K., Zeng L. Y., Han Z. X., Li Z. Y., Wang X. Y. *, and Xu K. L., Amino acid residue E543 in JAK2 C618R is a potential therapeutic target for myeloproliferative disorders caused by JAK2 C618R mutation. Arch Biochem Biophys. 2012, 528:57-66.

Wu Q. Y., Li F., Wang X. Y. *, and Chen Z. J., Impact of inter-subunit interactions on the dimeric arginine kinase activity and structural stability. Arch Biochem Biophys. 2011, 512:61-68.

Wu Q. Y., Jin K. Z., Li F., Hu Z. Q., and Wang X. Y.*, Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability. Int J Biol Macromol. 2011, 49:822-831.

Wang X. Y., Dutton R. J., Beckwith J., and Boyd D., Membrane topology and mutational analysis of Mycobacterium tuberculosis VKOR, a protein involved in disulfide bond formation and a homologue of human vitamin K epoxide reductase. Antioxid Redox Signal. 2011, 14:1413-1420.

Wang H. R., Zhu W. J., and Wang X. Y.*, Mechanism of inhibition of arginine kinase by flavonoids consistent with thermodynamics of docking simulation. Int J Biol Macromol. 2011, 49:985-991.

Jing X. L., Fan M. N., Jia G., Liu L. W., Ma L., Zheng C. C., Zhu X. P., Liu H. M., and Wang X. Y.*, A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana. J Virol. 2011, 85:12792-12803.

Feng W. K., Wang L., Lu Y., and Wang X. Y.*, A protein oxidase catalysing disulfide bond formation is localized to the chloroplast thylakoids. FEBS J. 2011, 278:3419-3430.

Li F., Wu Q. Y., Sun Y. L., Ma N. N., Wang X. Y., and Meng Q. W., Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability. Int J Biol Macromol. 2010, 46:350-355. Wu X. Q., Zhu W. J., Lu Z. R., Xia Y., Yang J. M., Zou F., and Wang X. Y.*, The effect of rutin on arginine kinase: inhibition kinetics and thermodynamics merging with docking simulation. Int J Biol Macromol. 2009, 44:149-155.

Wu X. Q., Xu H., Yue H., Liu K. Q., and Wang X. Y.*, Inhibition kinetics and the aggregation of alpha-glucosidase by different denaturants. Protein J. 2009, 28:448-456.

Wu Q. Y., Li F., and Wang X. Y.*, Val65 plays an important role in the substrate synergism, structural stability and activity of arginine kinase. Int J Biol Macromol. 2009, 45:393-398.

Shi X. W., Sun M. L., Zhou B., and Wang X. Y.*, Identification, characterization, and overexpression of a phytase with potential industrial interest. Can J Microbiol. 2009, 55:599-604.

Zhou J. P., Yang Z. B., Yang W. R., Wang X. Y., Jiang S. Z., and Zhang G. G., Effects of a New Recombinant Phytase on the Performance and Mineral Utilization of Broilers Fed Phosphorus-Deficient Diets. The Journal of Applied Poultry Research. 2008, 17:331.

Wu Q. Y., Li F., and Wang X. Y.*, Evidence that amino-acid residues are responsible for substrate synergism of locust arginine kinase. Insect Biochem Mol Biol. 2008, 38:59-65.

Wu Q. Y., Li F., and Wang X. Y.*, Evidence that the amino acid residue P272 of arginine kinase is involved in its activity, structure and stability. Int J Biol Macromol. 2008, 43:367-372.

Zhu W. J., Li M., and Wang X. Y.*, Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Int J Biol Macromol. 2007, 41:564-571.

Zhao D. M., Wang M., Mu X. J., Sun M. L., and Wang X. Y.*, Screening, cloning and overexpression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics. Lett Appl Microbiol. 2007, 45:522-528.

Xia Y., Park Y. D., Mu H., Zhou H. M., Wang X. Y.*, and Meng F. G., The protective effects of osmolytes on arginine kinase unfolding and aggregation. Int J Biol Macromol. 2007, 40:437-443.

Wu Q. Y., Li F., Zhu W. J., and Wang X. Y.*, Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis. Comp Biochem Physiol B Biochem Mol Biol. 2007, 148:355-362.

获奖记录

2011年获高等学校教学名师称号，曾获得山东省首届教学名师和山东农业大学学生心目中十大优秀教师称号 。

2011年9月8日，荣获"国家教学名师奖" 。

山东省首届"教学名师奖"。

王晓云教授2001年荣获全国农林院校优秀多媒体课件二等奖。

2002年获得第二届国家电子出版物奖提名奖。

2007年荣获山东农业大学学生心目中"十大优秀教师"荣誉称号。

2001年和2005年分别获得山东省教学成果二等奖和三等奖。

所在的教学团队为生物化学与分子生物学系列课程国家级教学团队。