李勁松

李勁松，中科院上海生科院生物化學與細胞生物學研究所研究員，研究組長，博士生導師。

李勁松
原圖鏈接 圖片來源中國科學院 中國科學院院士
出生	1971年10月14日 江西南昌
國籍	中國
母校	江西農業大學
職業	教育科研工作者

目錄

人物經歷

1993年7月畢業於江西農業大學畜牧獸醫系，獲理學學士學位;1996年7月畢業於揚州大學畜牧獸醫學院，獲理學碩士學位;2002年7月畢業於中國科學院動物研究所，獲理學博士學位;2002年8月至2007年7月在美國洛克菲勒大學從事博士後研究;2007年8月起任中科院上海生科院生物化學與細胞生物學研究所研究員，研究組長。

2021年11月18日，當選中國科學院2021年院士（生命科學和醫學學部）。

獲獎榮譽

2012年獲「傑出青年基金」支持，2013年入選「中青年科技創新領軍人才」。

研究方向

幹細胞與胚胎髮育

研究工作

2012年，研究組建立了來自小鼠精子的孤雄單倍體胚胎幹細胞，並證明這些細胞能夠代替精子使卵母細胞「受精」產生半克隆小鼠(稱為「半克隆」技術)(Yang et al., Cell, 2012)。然而，半克隆技術不成熟，健康半克隆小鼠出生率低(2%左右)。2015年，研究組獲得了能穩定高效產生半克隆小鼠的單倍體幹細胞(出生效率達到20%左右)，因此又稱為「人造精子細胞」(Zhong et al., Cell Stem Cell, 2015)。2018年，研究組通過結合「人造精子細胞」和CRISPR-Cas9介導的Base editor系統，實現蛋白質關鍵氨基酸的在體遺傳篩選(Li et al., Nature Cell Biology, 2018)。

所獲榮譽

2020年12月，被授予「上海市先進工作者」榮譽稱號。

代表論著

1，Li Y & Li J*. Technical advances contribute to the study of genome imprinting. PLOS Genet 2019. (Invited review)

2，Wang L & Li J*. 『Artificial spermatid』-mediated genome editing. Biol Reprod 2019. (Invited review)

3，Wang J#, Chen J#, Wu G#, Zhang H#, Du X#, Chen S, Zhang L, Wang K, Fan J, Gao S, Wu X, Zhang S, Kuai B, Zhao P, Chi B, Wang L, Li G, Wong CCL, Zhou Y*, Li J*, Yun C* & Cheng H*. NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction. Genes & Dev 2019 Mar 10. DOI: 10.1101/gad.322602.118

4，Song W, Wang R, Jiang W, Yin Q, Peng G, Yang R, Yu QC, Chen J, Li J, Cheung TH, Jing N & Zeng Y. Hormones induce the formation of luminal-derived basal cells in the mammary gland. Cell Res 2019 Jan 10.

5，Li J* & Gao C*. Preface to the special topic on genome editing research in China. Nat Sci Rev 2019

6，Jiang Jing, Yan M, Li D & Li J*. Genome tagging project: tag every protein in mouse through 「artificial spermatids」. Nat Sci Rev 2019

7，Lander E, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li J, Liu D, Naldini L, Nie JB, Qiu R, Schoene-Seifert B, Shao F, Terry S, Wei W & Winnacker EL. Adopt a moratorium on heritable genome editing. Nature 567(7747)(2019): 165-168.

8，Wang Haoyi, Li J, Li W, Gao C & Wei W. CRISPR twins: China Academy responds. Nature 564(7736)(2018): 345.

9，Li Qing, Li Y, Yang S, Huang S, Yan M, Ding Y, Tang W, Lou X, Yin Q, Sun Z, Lu L, Shi H, Wang H, Chen Y* & Li J*. CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol 20(11)(2018): 1315-1325. Epub 2018 Oct 01.

10，Chen Yao, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J*, Tang F* & Tong M*. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Res 28(9)(2018), 879-896. Epub 2018 Jul 30.

11，Qu Chao, Yan M, Yang S, Wang L, Yin Q, Liu Y, Chen Y & Li J*. Haploid embryonic stem cells can be enriched and maintained by simple filtration. J Biol Chem 293 (14) (2018): 5230-5235.

1,2，Li ZiYi, Huang M, Wang X, Zhu Y, Li J, Wong CCL & Fang Q. Nanoliter-scale oil-air-droplet chip-based single cell proteomic analysis. Anal Chem 90(8)(2018), 5430-5438. Epub 2018 Mar 27.

13，Liu Wuqing, Li S, Zhang Y, Li J & Li Y. Efficient CRISPR-based genome editing using tandem guide RNAs and editable surrogate reporters. FEBS Open Bio 8(7)(2018), 1167-1175.

14，Wang Xiukun & Li J*. An intermediate cell state allows rerouting of cell fate. J Biol Chem 292(46)(2017), 19133-19134. (Highlight).

15，Wei Leixin, Wang X, Yang S, Yuan W* & Li J*. Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene-editing. J Genet Genomics 44(9)(2017), 461-463. Aug 12.

李勁松
原圖鏈接 圖片來源搜狐 中國科學院院士 李勁松

1,6，Zheng Yi, Jongejan A, Mulder CL, Mastenbroek S, Repping S, Wang Y, Li J & Hamer G. Trivial role for NSMCE2 during in vitro proliferation and differentiation of male germline stem cells. Reproduction 154(3)(2017), 81-95.

17，Li Haisen, Guo A, Xie Z, Tu W, Yu J, Wang H, Zhao J, Zhong C, Kang J, Li J, Huang S & Shen L. Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation. Sci Rep 7(1)(2017), 13222.

1,8，Feng Su, Xing C, Shen T, Qiao Y, Wang R, Chen J, Liao J, Lu Z, Yang X, Abd-Allah SM, Li J, Jing N & Tang K. Abnormal paraventricular nucleus of hypothalamus and growth retardation associated with loss of nuclear receptor gene COUP-TFII. Sci Rep 7(1)(2017), 5282.

19，Wang Yinghua, Ding Y & Li J*. CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Methods Mol Biol 1622(2017), 293-305.

20，Zuo Erwei#, Cai Y-J#, Li K#, Wei Y#, Wang B-A#, Sun Y, Liu Z, Liu J, Hu X, Wei W, Huo X, Shi L, Tang C, Liang D, Wang Y, Nie Y-H, Zhang C-C, Yao X, Wang X, Zhou C, Ying W, Wang Q, Chen R-C, Shen Q, Xu G-L, Li J, Sun Q*, Xiong Z-Q* & Yang H*. One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs. Cell Res 27(7)(2017), 933-945. Epub 2017 June 6.

21，Gou Lantao, Kang J, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua M, Lu Y, Zhu Y, Li Z, Chen H, Wu L, Li D, Fu X, Li J, Shi H* & Liu M*. Ubiquitination-deficient mutations in human Piwi cause male infertility by impairing histone-to-protamine exchange during spermiogenesis. Cell 169(6)(2017), 1090-1104. Epub 2017 May 25.

22，Wu Keliang#, Zhong C#, Chen T#, Zhang X, Tao W, Zhang J, Li H, Zhao H*, Li J* & Chen ZJ*. Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy. Cell Res 27(8)(2017), 1069-1072. Epub 2017 May 12.

23，Wang Lingbo#, Li MY#, Qu C, Miao WY, Yin Q, Liao J, Cao HT, Huang M, Wang K, Zuo E, Peng G, Zhang SX, Chen G, Li Q, Tang K, Yu Q, Li Z, Wong C, Xu G, Jing N, Yu X* & Li J*. CRISPR-Cas9-mediated genome-editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development. Cell Res 27(6)(2017), 815-829. Epub 2017 April 21.

24，Xu He, Yue C, Zhang T, Li Y, Guo A, Liao J, Pei G, Li J* & Jing N*. Derivation of haploid neurons from mouse androgenetic haploid embryonic stem cells. Neurosci Bull 33(3)(2017), 361-364. Epub Mar 3.

25，Wu Keliang, Chen T, Huang S, Zhong C, Yan J, Zhang X, Li J, Gao Y & Chen ZJ. Mitochondrial replacement by pre-pronuclear transfer in human embryos. Cell Res 27(6)(2017), 834-837. Epub 2017 April 4.

26，Zhong Cuiqing & Li J*. Efficient generation of gene-modified mice by haploid embryonic stem cell-mediated semi-cloned technology. Methods Mol Biol 1498 (2017), 121-133.

27，Wang Xiongjun, Qiao Y, Xiao MM, Wang L, Chen J, Lv W, Xu L, Li Y, Wang Y, Tan MD, Huang C, Li J, Zhao TC, Hou Z, Jing N & Chin YE. Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells. Cell Rep 18(4)(2017), 933-946.

28，Yang Jiao, Guo R, Wang H, Ye X, Zhou Z, Dan J, Wang H, Gong P, Deng W, Yin Y, Mao S, Wang L, Ding J, Li J, Keefe DL, Dawlaty MM, Wang J, Xu G & Liu L. Tet enzymes regulate telomere maintenance and chromosomal stability of mouse ESCs. Cell Rep 15(8)(2016), 1-13. May 24, 2016.

29，Jin Lifang & Li J. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research 37(4)(2016), 205-213.

30，Burgress Shawn, Cheng L, Gu F, Huang L, Huang Z, Lin S, Li J, Li W, Qin W, Sun Y, Songyang Z, Wei W, Wu Q, Wang H, Wang X, Xiong JW, Xi J, Yang H, Zhou B & Zhang B. Questions about NgAgo. Protein Cell 7(12)(2016), 913-915.

31，Zhong C #, Zhang M#, Yin Q, Zhao H, Wang Y, Huang S, Tao W, Wu K*, Chen Z-J* & Li J*. Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus. Cell Res 26(6)(2016), 743-746. Epub 2016 May 17.

32，Bai M, Liang D, Wang Y, Li Q, Wu Y & Li J*. Spermatogenic cell-specific gene mutation in mice via CRISPR-Cas9. J Genet Genomics 43(5)(2016), 289-296. Epub 2016 Feb 26.

33，Bai M, Wu Y & Li J*. Generation and application of mammalian haploid embryonic stem cells. J Intern Med 280(3)(2016), 236-245. (Invited Review).

34，Zhong C#, Xie Z#, Yin Q#, Dong R, Yang S, Wu Y & Li J*. Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection. Cell Res 26(1)(2016), 131-134. Epub 2015 Nov 17.

35，Zhong C#, Yin Q#, Xie Z#, Bai M#, Dong R#, Tang W, Xing Y, Zhang H, Yang S, Chen L-L, Bartolomei MS, Ferguson-Smith A, Li D, Yang L*, Wu Y* & Li J*. CRISPR-Cas9-mediated genetic screening in mice with haploid embryonic stem cells carrying a guide RNA library. Cell Stem Cell 17(2)(2015), 221-232.

36，Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna J, Feng G, Greely HT, Isasi R, Ji W, Kim J, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS, Moreno J, Naldini L, Pera M, Perry A, Venter JC, Zhang F & Zhou Q. CRISPR germline engineering-the community speaks. Nat Biotechnol 33(5)(2015), 478-486. (Feature).

37，Tong M* & Li J*. Similarity of epigenetic reprogramming in primordial germ cells between human and mouse. Natl Sci Rev 2015, 2(4)(2015), 384. (Invited Research Highlights)

38Liu P, Dou X, Liu C, Wang L, Xing C, Peng G, Chen J, Yu F, Qiao Y, Song L, Wu Y, Yue C, Li J, Han J, Tang K & Jing N. Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. Nat Commun 6(2015), 6830.

39，Qin Y, Qin J, Zhou C, Li J & Gao W. Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer. Cell Cycle 14(8)(2015), 1282-1290.

40，Zhu W, Yao X, Liang Y, Liang D, Song L, Jing N, Li J & Wang G*. Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling. Development 142(3)(2015), 465-476. Epub Jan 6. pii: dev.112946.

41，Wu Y#, Zhou H#, Fan X#, Zhang Y#, Zhang M#, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L*, Tang F* & Li J*. Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Res 25(1)(2015), 67-79. Epub 2014 Dec 5.

42，Li J*. Stem cell, basis and application. Sci Bull 60(20)(2015), 1711-1712. (組織專刊並撰寫序言).

43，李勁松. 基因編輯技術—後基因組時代生命科學研究的助力器. 生命科學（基因編輯專刊） 27(1)(2015), 1. （組織專刊並撰寫序言）

44，Lian X， Xu J， Li J & Chien K. Next-generation models of human cardiogenesis via genome editing. Cold Spring Harb Perspect Med 4(12)(2014), a013920. doi: 10.1101/cshperspect.a013920.

45，Guo F#, Li X#, Liang D#, Li T#, Zhu P, Guo H, Wu X, Wen L, Gu T, Hu B, Walsh CP, Li J*, Tang F* & Xu G*. Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote. Cell Stem Cell 15(4)(2014), 447-458. [Epub ahead of print].

46，Zhang M, Zhou H, Zheng C, Xiao J, Zuo E, Liu W, Xie D, Shi Y, Wu C, Wang H, Li D & Li J*. The roles of testicular c-kit positive cells in de novo morphogenesis of testis. Sci Rep 4(2014), 5936. doi: 10.1038/srep05936.

47，Zhu Q, Song L, Peng G, Sun N, Chen J, Zhang T, Sheng N, Tang W, Qian C, Qiao Y, Tang K, Han JD, Li J & Jing N. The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. Elife 14(2014), e02224. doi: 10.7554/eLife.02224. [Epub ahead of print].

48，周海，伍春蓮*，李勁松*. 不同年齡小鼠精原幹細胞系的建立. 中國細胞生物學學報 36(6)(2014), 752-757.

49，Zhao B, Yang D, Jiang J, Li J, Fan S, Huang M, Fan Y, Jin Y & Jin Y. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies. BMC Genomic 15(2014), 488. doi: 10.1186/1471-2164-15-488.

50，Yu Y, Liang D, Tian Q, Chen X, Jiang B, Chou BK, Hu P Cheng L, Gao P, Li J & Wang G. Stimulation of somatic cell reprogramming by Eras-Akt-Foxo1 signaling axis. Stem Cells 32(2)(2014), 349-363. doi: 10.1002/stem.1447. [Epub ahead of print].

51，Qin Y, Zhou P, Zhou C, Li J & Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev 23(4)(2014), 363-371. W 52，u Y#, Liang D#, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D & Li J*. Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell 13(6)(2013), 659-662.

53，Yang H#, Liu Z#, Ma Y#, Zhong C, Yin Q, Zhou C, Shi L, Cai Y, Zhao H, Wang H, Tang F, Wang Y, Zhang C, Liu X, Lai D, Jin Y*, Sun Q* & Li J*. Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. Cell Res 23(10)(2013), 1187-1200.

54，Qin Y#, Lin J#, Zhou C#, Yin Q, Xie Z, Zhang X, Liu X, Gao W & Li J*. Mice cloned from white adipose tissue-derived cells. J Mol Cell Biol 5(5)(2013), 348-350.

55，Jiang J#, Lv W#, Ye X, Wang L, Zhang M, Yang H, Okuka M, Zhou C, Zhang X, Liu L* & Li J*. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Res 23(1)(2013), 92-106.

56，Zhao S#, Gou LT#, Zhang M#, Zu LD, Hua MM, Hua Y, Shi HJ, Li Y, Li J, Li DS, Wang ED* & Liu M. piRNA-triggered MIWI ubiquitination and removal by APC/C in late spermatogenesis. Dev Cell 24(1)(2013), 13-25.

57，Shi L, Yang H & Li J*. Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses. Protein & Cell 3(11)(2012), 806-810. (Invited review)

58，Yang H#, Shi L#, Wang B#, Liang D, Zhong C, Liu W, Nie Y, Liu J, Zhao J, Gao X, Li D, Xu G-L* & Li J*. Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. Cell 149(3)(2012), 605-617.

59，Ma Yu, Gu J, Li C, Wei X, Tang F, Shi G, Jiang J, Kuang Y, Li J, Wang Z, Xie X & Jin Y. Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells. Stem Cell Res Ther 3(4)(2012), 29.

60，Gu T#, Guo F#, Yang H#, Wu HP, Xu GF, Liu W, Xie ZG, Shi L, He X, Jin SG, Iqbal K, Shi YG, Deng Z, Szabo PE, Pfeifer GP, Li J* & Xu GL*. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477(7366)(2011), 606-610.

61，Jiang J#, Ding G#, Lin J#, Zhang M, Shi L, Lv W, Yang H, Xiao H, Pei G, Li Y, Wu J* & Li J*. Different developmental potential of pluripotent stem cells generated by different reprogramming strategies. J Mol Cell Biol 3(3)(2011), 197-199.

62，Lin J, Shi L, Zhang M, Yang H, Qin Y, Zhang J, Gong D, Zhang X, Li D & Li J*. Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer. Cell Stem Cell 8(4)(2011), 371-375.

63，Yang H#, Shi L#, Chen D & Li J*. Mice generated after round spermatid injection into haploid two-cell blastomeres. Cell Res 21(5)(2011), 854-858.

64，Li X, Zhu L, Yang A, Lin J, Tang F, Jin S, Wei Z, Li J & Jin Y. Calcineurin-NFAT signaling critically regulates early lineage specification in mouse embryonic stem cells and embryos. Cell Stem Cell 8(1)(2011), 46-58.

65，Wang Y, Chen J, Hu J, Wei X, Qin D, Gao J, Zhang L, Jiang J, Li J, Liu J, Lai K, Kuang X, Zhang J, Pei D & Xu G. Reprogramming of mouse and human somatic cells by high-performance engineered factors. EMBO Rep 12(4)(2011), 373-378.

66，Yang H, Shi L, Zhang S, Lin J, Jiang J & Li J*. High-efficiency somatic reprogramming induced by intact MII oocytes. Cell Res 20(9)(2010), 1034-1042.

67，Li L, Sun L, Gao F, Jiang J, Li C, Gu J, Wei Z, Yang A, Lu R, Ma Y, Tang F, Kwon SW, Zhao Y, Li J & Jin Y. Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation. Proc Natl Acad Sci USA 107(4)(2010),1402-1407.,

68，Chen T, Yuan D, Wei B, Jiang J, Kang J, Ling K, Gu Y, Li J, Xiao L & Pei G. E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation. Stem Cells 28(8)(2010), 1315-1325.

69，Zhu Z, Wang Y, Li X, Wang Y, Xu L, Wang X, Sun T, Dong X, Chen L, Mao H, Yu Y, Li J, Chen PA & Chen CD. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis. Cell Res 20(7)(2010), 794-801.

70，Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, Yang Y, Jin S, Wei Z, Jiang H, Li J & Jin Y. Germline-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor. PLos One 4(8)(2009), e6724.

71，Huang J, Chen T, Liu X, Jiang J, Li J, Li D, Liu X, Li W, Kang J & Pei G. More synergetic cooperation of Yamanaka factors in induced pluripotent stem cells than in embryonic stem cells. Cell Res 19(10)(2009), 1127-1138.

72，Li J & Mombaerts P. Nuclear transfer-mediated rescue of the nuclear genome of nonviable mouse cells frozen without cryoprotectant. Biol Reprod 79(4)(2008), 588-593.

73，Li J#, Guasch G#, Greco V#, Fuchs E* & Mombaerts P*. Cloned mice from Skin Stem Cells. Proc Natl Acad Sci USA 104(8)(2007), 2738-2743.

74，Li J, Ishii T, Wen D & Mombaerts P. Non-equivalence of cloned and clonal mice. Curr Biol 15(18)(2005), R756-R757.

75，Li J, Ishii T, Feinstein P & Mombaerts P. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981)(2004), 393-398.

76，Han Z, Chen D, Li J, Sun Q, Wan Q, Kou Z, Rao G, Lei L, Liu Z & Fang S. Mitochondrial DNA heteroplasmy in calves cloned by using adult somatic cell. Mol Reprod Dev 67(2)(2004), 207-214

77，Chen D#, Li J#, Han Z, Lei L, Liu Z, Kou Z, Ma S, Du Q & Sun Q. Somatic cell bovine cloning: efficiency of donor cells from female and male using different recipient cows. Chin Sci Bull 48(6)(2003), 549-554.

78，Zhu Z, Chen D, Li J, Lian L, Lei L, Han Z & Sun Q. Rotation of meiotic spindle is controlled by microfilaments in mouse oocytes. Biol Reprod 68(3)(2003): 943-946.

79，Han Z, Chen D, Li J, Sun Q, Wang P, Du J & Zhang H. Flow cytometric cell-cycle analysis of cultured fibroblasts from the giant panda, Ailuropoda melanoleuca L. Cell Biol Int 27(4)(2003), 349-353.

80，Wen D, Yang C, Cheng Y, Li J, Liu Z, Sun Q, Zhang J, Lei L, Wu Y, Kou Z & Chen D. Comparison of developmental capacity for intra- and interspecies cloned cat (Felis catus) embryos. Mol Reprod Dev 66(1)(2003), 38-45.

81，Chen Y, He Z, Liu A, Wang K, Mao W, Chu J, Lu Y, Fang Z, Shi Y, Yang Q, Chen D, Wang M, Li J, Huang S, Kong X, Shi Y, Wang Z, Xia J, Long Z, Xue Z, Ding W & Sheng H. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes. Cell Res 13(4)(2003), 251-263.

82，Li J, Chen D, Han Z, Zhu Z, Wen D, Liu Z, Wang M, Lian L, Du J, Wang P & Zhang H. Serial Nuclear Transfer Improves the Development of Interspecies Reconstructed Giant Panda Embryos. Chin Sci Bull 47(6)(2002), 467-469.

83，Chen D, Wen D, Zhang Y, Sun Q, Han Z, Liu Z, Shi P, Li J, Xianyu J, Lian L, Kou Z, Wu Y, Chen Y, Wang P & Zhang H. Interspeices implantation and mitochondria fate of panda-rabbit cloned embryos. Biol Reprod 67(2)(2002), 637-642.

84，Li J & Chen D. Cell cycle interactions between donor nucleus and recipient cytoplasm in mammalian nuclear transfer. Progress in Biochemistry and Biophysics 29(2) (2002), 206-210. (中文綜述)

85，Han Z, Chen D, Li J, Sun Q, Wang P, Huang Y & Du J. The culture of fibroblasts from diaphragm of Giant Panda. In Vitro Cell Dev Biol Anim 37(10)(2001), 644-645.

86，Li G, Chen D, Lian L, Sun Q, Wang M, Liu J, Li J & Han Z. Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplamic sperm injection (ICSI). Mol Reprod Dev 58(2)(2001), 180-185.

87，Ding B, Shi P, Xianyu J, Zhang Y, Chen D, Sun Q, Li G, Wang M, Liu J, Han Z, Song X, Li J & Chen Y. Microsatellite DNA analysis prove nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda. Chin Sci Bull 45(20)(2000), 1883-1885.

88，Li J, Zhuang D, Sun Q & Chen D. New advances in animal transgenic technology. Progress in Biochemistry and Biophysics 27(2) (2000), 124-126. (中文綜述),

89，Liu J, Wang M, Lian L, Li J, Gao S, Han Z, Sun Q, Song X, Zhang D, Li Y, Xu Z & Chen D. Nuclear Transfer Using Nonquiescent Adult Fibroblasts from a Bovine Ear. Chin Sci Bull 44(21)(1999), 1971-1974.

90，Chen D, Sun Q, Liu J, Li G, Lian L, Wang M, Han Z, Song X, Li J, Sun Q, Chen Y, Zhang Y & Ding B. The Giant Panda Somatic Nucleus can dedifferentiate in Rabbit Ooplasm and Support Early Development of the Reconstructed Egg. Sci China C Life Sci 42(4)(1999), 346-353 ,